Trypan blue Supplier Manufacturer in Chennai India
Chennai Chemical industry Based in Chennai India , Chennai chemical are a leading Chemical Manufacturer and Supplier of Trypan blue in Chennai India . We are supplied into various industrial markets including Household ,Institutional Cleaning, Personal Care and Industrial sectors including Oil Field, Agriculture, Lubricants, Metal Working chemical industry and Coatings markets.
Trypan blue is an azo dye that is used as a dye-stuff. It is a direct dye for cotton textiles. In biosciences, it is used as vital stain to selectively colour dead tissues or cells blue.
Live cells or tissues with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells appear as a distinctive blue colour under a microscope. Since live cells are excluded from staining, this staining method is also described as a dye exclusion method.
uses
Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. The method cannot distinguish between necrotic and apoptotic cells.It may be used to observe fungal hyphae and stramenopiles.Trypan blue is also used in ophthalmic cataract surgery to stain the anterior capsule in the presence of a mature cataract, to aid in visualization, before creating the continuous curvilinear capsulorhexis.
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